Immobilisation of thiabendazole specific antibodies on an agarose matrix for application in immunoaffinity chromatography†

Abstract

The covalent immobilisation of two monoclonal antibodies (each of which binds both thiabendazole and its metabolite, 5-hydroxythiabendazole) on an agarose matrix is described. Immunoaffinity columns, made using these immunosorbents, bound radiolabelled thiabendazole and a number of eluents, were evaluated, with ethanol–water (70%) being selected as the most suitable. Re-equilibration of the immunosorbent was possible by purging with water, resulting in a reusable column. The columns were found to be stable under the conditions employed.