Immobilisation of thiabendazole specific antibodies on an agarose matrix for application in immunoaffinity chromatography†
The covalent immobilisation of two monoclonal antibodies (each of which binds both thiabendazole and its metabolite, 5-hydroxythiabendazole) on an agarose matrix is described. Immunoaffinity columns, made using these immunosorbents, bound radiolabelled thiabendazole and a number of eluents, were evaluated, with ethanol–water (70%) being selected as the most suitable. Re-equilibration of the immunosorbent was possible by purging with water, resulting in a reusable column. The columns were found to be stable under the conditions employed.