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Issue 3, 1999
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Rapid determination of lactulose in milk by microdialysis and biosensors

Abstract

A simple and rapid flow system for the determination of lactulose in milk samples was developed. It is based on the hydrolysis of lactulose to galactose and fructose by the enzyme β-galactosidase immobilised in a reactor. The amount of fructose produced was measured with an electrochemical biosensor based on the fructose dehydrogenase enzyme, K3[Fe(CN)6] as mediator and a platinum based electrochemical transducer. Parameters such as the enzyme immobilisation in the reactor and under the electrode surface, the lifetime of the β-galactosidase reactor and of the dehydrogenase biosensor and the flow parameters were studied and optimised. Fructose was determined in the range 1 × 10–6–5 × 10–3 mol l–1 with an RSD of about 2% and a detection limit of 5 × 10–7 mol l–1. The use of a microdialysis probe as the sampling system permitted the direct measurement of lactulose in milk samples without pre-treatment in the range 1 × 10–5–5 × 10–3 mol l–1. The sensitivity of the procedure allowed pasteurised, UHT and in-container sterilised milk to be distinguished.

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Article information


Analyst, 1999,124, 325-329
Article type
Paper

Rapid determination of lactulose in milk by microdialysis and biosensors

D. Moscone, G. Palleschi, R. A. Bernardo, E. Marconi and A. Amine, Analyst, 1999, 124, 325
DOI: 10.1039/A808535H

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