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Issue 6, 1999
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Identification of the principal selenium compounds in selenium-enriched natural sample extracts by ion-pair liquid chromatography with inductively coupled plasma- and electrospray ionization-mass spectrometric detection

Abstract

Selenium-enriched garlic and yeast sample extracts and digests were analyzed using ion-pair high performance liquid chromatography (HPLC) with on-line inductively coupled plasma-mass spectrometric (ICP-MS) and electrospray ionization-mass spectrometric (ESI-MS) detection. The principal selenium compounds in these samples were identified as selenomethionine, and Se-adenosyl-selenohomocysteine in yeast, and γ-glutamyl-Se-methyl-selenocysteine and possibly γ-glutamyl-selenomethionine in garlic. The compounds identified account for 85 and 90% of the total selenium content of the yeast and the garlic samples, respectively. On-line HPLC-ESI-MS selected ion chromatograms (SIC) and mass spectra of selenium compounds extracted from selenium enriched samples are presented. Limits of quantification (LOQ, defined as S/N = 10) for HPLC-ICP-MS were in the range 10–50 ng mL–1 Se in the injected extracts. LOQ values for HPLC-ESI-MS were ca. 100 times higher than those of HPLC-ICP-MS.

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Article type: Paper
DOI: 10.1039/A902770J
Anal. Commun., 1999,36, 249-252

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    Identification of the principal selenium compounds in selenium-enriched natural sample extracts by ion-pair liquid chromatography with inductively coupled plasma- and electrospray ionization-mass spectrometric detection

    M. Kotrebai, J. F. Tyson, P. C. Uden, M. Birringer and E. Block, Anal. Commun., 1999, 36, 249
    DOI: 10.1039/A902770J

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