Direct electrochemistry of horseradish peroxidase adsorbed on glassy carbon electrode from organic solutions

Abstract

Using a ‘solvent engineering’ approach, the direct electrochemistry of catalytically active horseradish peroxidase (HRP) adsorbed on glassy carbon electrodes from HRP–DMSO and HRP–formamide solutions has been readily obtained in aqueous phosphate buffer (pH 7.0), with a surface formal potential of -0.365 V vs. Ag/AgCl and an apparent electron transfer rate constant of 0.655 s ^{- 1} .