Biosynthetic origin of C-26 and C-27 of the phytoecdysteroids cyasterone and 29-norcyasterone in Ajuga hairy roots
Abstract
Feeding of [13C2] acetate to the hairy root culture of Ajuga reptans var. atropurpurea and 13C NMR analysis of the biosynthesized cyasterone and 29-norcyasterone reveal that the lactone carbonyl carbon of these phytoecdysteroids is derived from C-2 of mevalonate, whereas the methyl group on C-25 comes from C-6.