Rapid fluorescence enzyme linked immunosorbent assay for subtilisin

Abstract

A simple and rapid ELISA with a fluorescent end-point has been developed for the measurement of subtilisin. The ELISA can be applied to the analysis of enzyme in solutions that have been eluted from filters following the capture of air samples in the workplace. The assay has adequate precision and shows no interference from other enzymes tested. A comparison has been made between this assay and a similar ELISA method using spectrophotometric detection. The use of fluorescence detection reduces the over-all assay time from 60 to 25 min and reduces the concentrations of antibody reagents required in the assay. The assay described is quicker and more sensitive than the current methods in use.