Determination of creatinine in human serum. Statistical intercalibration of methods

Abstract

An intercalibration of methods was performed in the determination of creatinine in human plasma. Jaffè, enzymatic creatinine-deaminase (CRDI) and ion-interaction reagent high-performance liquid chromatography (HPLC) methods were employed and the results were compared using a parametric t-test for multiple samples and non-parametric Mann–Whitney, Wilcoxon and Friedman tests. The comparison did not show a statistically significant difference in the use of the three methods. The ion-interaction HPLC procedure is proposed as a reference method for creatinine assays.