Cyclisation of N-alkylmaleamic acids mediated by acetic anhydride in dimethylacetamide in the presence of traces of cobalt naphthenate has been used for efficient assembly of a range of fluorescent maleimide reagents. The fluorescence responses of these reagents to addition of thiol across the maleimide double bond, and to hydrolysis of the maleimide ring, are described.
J. E. T. Corrie, J. Chem. Soc., Perkin Trans. 1, 1994, 2975 DOI: 10.1039/P19940002975
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