Design and properties of new 19F NMR Ca2+ indicators: modulation of the affinities of BAPTA derivatives via alkylation
Abstract
The effects of alkyl substitution in the aminodiacetic groups of 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) have been examined systematically with the aim of increasing the affinity for Ca2+ of novel 19F NMR and fluorescent Ca2+ indicators based on the BAPTA structure into the range required to measure the cytosolic free Ca2+ concentration ([Ca2+]i). Single methylation on the β-carbon of each aminodiacetic acid group of BAPTA derivatives gave increases in the association constant for Ca2+[K(Ca2+)] of up to 22-fold, depending on the structure of the parent BAPTA derivative. This was shown to be the most effective alkylation for BAPTA derivatives to raise K(Ca2+) with a minimal increase in pKa and no loss of selectivity for Ca2+, so that the insensitivity of Ca2+ binding to pH and [Mg2+] over the physiological ranges was maintained.
(1) Single methylation on the β-carbon of each aminodiacetic acid group of BAPTA derivatives symmetrically substituted with single fluorines at the 4-position (4FBAPTA) or at the 5-position (5FBAPTA) raised log K(Ca2+) from 5.61 to 6.81 and 6.12 to 7.47, respectively. The corresponding pKav values (average of the two highest pKa, values in the molecule) were increased from 4.50 to 6.02 and 5.85 to 6.59. The 19F NMR spectroscopic properties of the methylated derivatives were unchanged compared with the parent compounds except for slower exchange rates. These new methylated derivatives of 4FBAPTA and 5FBAPTA therefore extend the range of K(Ca2+) available for 19F NMR indicators of [Ca2]i.
(2) Single methylation on the β-carbon of each aminodiacetic acid group of BAPTA derivatives symmetrically substituted at the 5-position on the aromatic rings with trifluoromethyl groups increased log K(Ca2+) from 4.83 to 6.14 and the pKav from 5.39 to 5.90, respectively. There is a Ca2+-induced chemical shift of the 19F NMR resonance of 0.81 ppm upfield with slow exchange at 376 MHz and 30 °C. This compound was selected as a prototype for 19F NMR indicators with enhanced sensitivity through the incorporation of two trifluoromethyl groups to replace the two single fluorine substituents in the current 19F NMR indicators.