A highly sensitive post-column reaction detection system was developed for the liquid chromatographic determination of non-reducing carbohydrates. Non-reducing carbohydrates were oxidized by periodate prior to forming fluorescent compounds by reaction with guanidine in alkaline solution. The calibration graphs were linear between 50 pmol and 10 nmol.
S. Yamauchi, C. Nakai, N. Nimura, T. Kinoshita and T. Hanai, Analyst, 1993, 118, 769 DOI: 10.1039/AN9931800769
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