Isolation of new isocyanide metabolites of Trichoderma hamatum as their (η5-pentamethylcyclopentadienyl)- or (η5-ethyltetramethylcyclopentadienyl)-di-µ-thiocyanato-rhodium complexes
Abstract
Many isolates of Trichoderma spp. have been shown to produce 10–20 unstable metabolites, characterized by the presence of an isocyano group, by a recently described analytical method. The purification of these metabolites can be followed by this method and in this way 4-hydroxy-8-isocyano-1-oxaspiro[4·4]cyclonon-8-en-2-one 9, 1-(2,3-epoxy-1-hydroxy-3-isocyanocyclopent-4-enyl)ethanol 13, and 1-(1,4,5-trihydroxy-2-isocyanopent-2-enyl)ethanol 11 have been isolated and characterized. The presence of an isomer of methyl 3-(1,5-dihydroxy-3-isocyanocyclopent-3-enyl)prop-2-enoate 6, previously reported has been confirmed.