Synthesis of a disulphide linked heterotrimeric active site peptide segment of laminin

Abstract

A disulphide linked 95-mer heterotrimeric active site segment of laminin (B2 chain Met 1538 replaced with Nle) was synthesized by the solid phase peptide synthesis method utilizing the two-step trimethylsilyl bromide–HF deprotection procedure and the interlinking of the three subunits by the stepwise selective formation of two disulphide bridges using air-oxidation and thallium(III) trifluoroacetate oxidation.