Use of electrospray mass spectrometry in enzymic studies on acyl carrier protein implicated in fatty acid biosynthesis in Saccharopolyspora erythraea

Abstract

Two types of experiments are used to confirm that an acyl carrier protein (ACP) is involved in fatty acid biosynthesis using a cell-free extract of Saccharopolyspora erythraea: turnover using a chemically prepared acyl derivative of the ACP, and isolation of acylated intermediates produced by the action of the fatty acid synthase were detected by electrospray mass spectrometry.