Hypervalent iron-oxo porphyrin cation radical formation on reaction of H2O2 with the cytochrome-c-derived haem octapeptide microperoxidase-8 (MP-8) in aqueous solution

Abstract

The reaction between microperoxidase-8 (MP-8) and H₂O₂ has been investigated in aqueous buffer solution using the UV-vis spectrophotometric probe 2,2′-azino-bis(3-ethylbenthiazoline-6-sulphonic acid)(ABTS); evidence is presented for the intermediate formation of a hypervalent iron-oxo radical species analogous to compound I of the peroxidase enzymes.