Speciation of vanadium present in a model yeast system
Yeast cells were used as a model system for the investigation and identification of toxic forms of vanadium obtained on dosing a nutrient medium with various known vanadium species. Total vanadium uptake by the yeast cells was in the region of 10 p.p.m. when the nutrient medium was dosed with 200 p.p.m. of the toxic species VV prepared as a V2O5-NaOH solution. No cell growth was observed in the nutrient medium when the concentrations were above 200 p.p.m. However, vanadium was found to accumulate in the yeast cells to a much greater extent when the nutrient medium was dosed with solutions of the less toxic form VIV. Total vanadium in the cells was determined using flame atomic absorption spectrometry.