Iodimetric method for the determination of glucose with vanadium(V) in perchloric acid

Abstract

Ammonium (or sodium) vanadate in dilute perchloric acid was found to be an excellent reagent for the determination of glucose. The method involves refluxing a mixture containing glucose and an excess of vanadate solution in dilute perchloric acid over a small flame for 40 min. To this reaction mixture potassium iodide was added to determine the residual vanadate iodimetrically. The amount of glucose can then be determined by standardising the vanadate solution with either sodium thiosulphate or glucose solutions of known strengths; the latter gave consistently better results.