High-performance liquid chromatography of acidic dyes on a dynamically modified polystyrene-divinylbenzene packing material with multi-wavelength detection and absorbance ratio characterisation

Abstract

A chromatographic system utilising a dynamically modified 5-µm polystyrene-divinylbenzene packing material for the analysis of acidic dyes under isocratic conditions is described. When used in conjunction with a multi-wavelength UV-visible diode array detector an absorbance ratio technique (absorbance ratioing) provided a method for the characterisation of the 52 acidic dyes chromatographed under these conditions.