Determination of chlortetracycline residues in tissues using high-performance liquid chromatography with fluorescence detection

Abstract

A method is described for the determination of chlortetracycline residues in tissue samples. The samples were extracted into a hydrochloric acid-glycine solution and the extracts concentrated and purified on cyclohexyl-bonded reversed-phase cartridges. Any chlortetracycline present was converted to iso-chlortetracycline at pH 12, which was then separated from interfering compounds on a reversed-phase polymeric column using high-performance liquid chromatography with fluorescence detection. The detection and determination limits of the assay were 20 and 50 ng g^–1, respectively, making it suitable for statutory residue testing purposes.