Liquid chromatographic and fluorescent derivative aerobic degradation studies of dehydroascorbic acid in aqueous solution at elevated temperatures

Abstract

The aqueous degradation of dehydroascorbic acid (DHA) has been studied in the temperature range 52–90 °C. The DHA was determined by reversed-phase liquid chromatography and by derivatisation of DHA with o-phenylenediamine to form the fluorescent quinoxaline. The pseudo-first-order degradation of DHA has been verified and rate constants for the process are presented. The role of DHA in the degradation of ascorbic acid and previous DHA solution stability studies are discussed.