Chemistry of biotin: access to 2,3- and 5,6-didehydrobiotin derivatives
Abstract
Dehydration of the N1′-acetylbiotin methyl ester sulphoxides (3a) and (3b) under a variety of conditions affords the didehydrobiotin derivatives (4) and (5a). 5,6-Didehydrobiotin (5b) is effective as a biotin replacement factor for a bioA mutant of E. coil.