Fluorimetric determination of human serum albumin with Eriochrome Cyanine R
Abstract
A sensitive spectofluorimetric method has been developed for the determination of human serum albumin (HSA). The complex of Eriochrome Cyanine R (ECR) with HSA fluoresces at 423 nm (with excitation at 308 nm). The limit of determination is 0.5 µg ml–1 and the relative standard deviation is less than 2%. The composition of the fluorescent complex is 1 : 4 (HSA : ECR).