Determination of glucose in clinical samples by flow reversal flow injection analysis

Abstract

A new type of flow injection analysis system for multiple detection has been developed, in which the injected samples are first pumped to a detector and subsequently pumped to the same detector again by a reversed flow. Although with this system at least two pieces of information per sample are obtained, only one pump and one detector are needed, so the apparatus is relatively simple. Moreover, the dispersion of the samples is not greater than that with the normal one-way flow. The system was applied to the determination of glucose in clinical samples with a two-point rate assay. The reproducibility was also satisfactory (RSD = 2.1%, n= 7, for a standard sample).