Determination of glucosinolate in rapeseed meal by infrared spectrometry

Abstract

A method is proposed for the determination of glucosinolate in rapeseed meal by infrared spectrometry. Glucosinolate has a strong absorption peak at 800 cm^–1. As rapeseed oil and meal do not absorb at 800 cm^–1, they do not interfere in the determination at this wavelength. The relationship between the absorption intensity and concentration of glucosinolate obeyed Beer's law in the range 0.0–43.8 µg. A concentration of 2.4–4.8 µmol of glucosinolate per gram of meal can be detected in this way. One quarter to half of a grain of rapeseed is sufficient to determine glucosinolate directly.