Determination of bromazepam and its urinary metabolites, with a previous hydrolysis reaction, by voltammetric and spectrophotometric techniques

Abstract

Bromazepam (I) is hydrolysed in 3 M perchloric acid by refluxing for 45 min to give 2-amino-5-bromobenzoylpyridine (II). The cyclic and differential-pulse voltammetric behaviour of II on a rotating disc gold electrode was studied. Spectrophotometric and voltammetric methods for the determination of bromazepam, based on the hydrolysis reaction to II, were developed and applied to the analysis of pharmaceuticals and the determination of bromazepam and its metabolites in urine.