Concentration effects in exclusion chromatography. Quantitative prediction in θ-polymeric systems

Abstract

The concentration effects on the elution volume in theta binary and ternary polymeric systems have been studied by size-exclusion chromatography (s.e.c.). The experimental results have been compared with the predicted ones from a simple theoretical model mainly governed by the Huggins' coefficient, k. Different problems in the evaluation of k for ternary polymeric systems are described through general expressions relating this value with the viscometric expansion coefficient, α³_η. Finally, different ways to evaluate k have been tested, through comparison between predicted results from our model and experimental data from literature.