Determination of B2 vitamers in the body fluid of plankton using high-performance liquid chromatography with fluorescence detection

Abstract

A sensitive method, using high-performance liquid chromatography (HPLC) with fluorescence detection, has been developed for the separation and determination of B₂ vitamers in the body fluid of plankton. B₂ vitamers are liberated from the protein moiety in the body fluid by non-hydrolytic extraction with trichloroacetic acid and analysed by the proposed HPLC method. The major B₂ vitamer in the body fluid of plankton is riboflavin, although there is also evidence to suggest the presence of endogenous enzymes that can decompose flavin mononucleotide and flavin adenine dinucleotide.