Biosynthesis of the polyether antibiotic ICI139603 in Streptomyces longisporoflavus: investigation of deuterium retention after incorporation of CD313CO2H, 13CD3CO2H, and CH3CD213CO2H using 2H n.m.r. and edited 13C n.m.r. spectra

Abstract

The mechanism of carbocyclic ring formation in the biosynthesis of the polyether antibiotic ICI139603 (2) was investigated by deuterium retention studies after incorporation of CD₃¹³CO₂H, ¹³CD₃CO₂H, and CH₃CD₂¹³CO₂H using ²H n.m.r. spectroscopy, α- and β-isotopic shifts, and edited ¹³C n.m.r. spectroscopy.