Analytical investigation of some fluorogenic reactions of indol-3-yl acids with o-phthalaldehyde. Part I. Solution fluorimetric studies

Abstract

An investigation of the parameters that may affect the fluorogenic reaction of some indol-3-yl acids with o-phthalaldehyde in sulphuric acid medium has been carried out. Indol-3-ylacetic acid, a compound of biological interest, which was used as a model compound, was carefully examined together with seven other similar compounds. The influence of the concentration of sulphuric acid, oxygen, temperature, interferences and ultraviolet radiation was investigated. In the last instance, a beneficial effect on the fluorescence signal was observed with most compounds and this led to the proposal of a photochemical-fluorimetric method of analysis.