Fluorimetric determination of glibenclamide in plasma using pyrene-1-aldehyde

Abstract

A method is described for the fluorimetric determination of glibenclamide in plasma by its reaction with pyrene-1-aldehyde. The procedure is suitable for routine use with diabetic patients receiving commercially available formulations of the drug. Calibration graphs are linear over the range 50–500 ng of glibenclamide. The limit of detection is about 10 ng ml^–1 in plasma, and the relative standard deviation is 5.0–5.7% for 125 ng ml^–1 in plasma. Within the limits of the assay procedure, plasma blank values are independent of plasma volume.