Combined use of multiple and selective proton-decoupled 13C and 1H n.m.r. spectra for complete proton and carbon-13 resonance assignments of polypeptides
Abstract
Selective proton irradiations during proton and carbon-13 n.m.r. observation demonstrate unequivocal assignments of 1H and 13C resonances of the model peptide valinomycin and the usefulness of this technique in peptide sequencing is noted.