Histidine H-2 n.m.r. resonances of sperm whale oxy-, carbonyl-, and met-myoglobin

Abstract

Seven H-2 histidine n.m.r.resonances of sperm whale metmyoglobin and eleven resonances of both carbonyl- and oxy-myoglobin are assigned and pK′ values determined; there are six titration curves from surface histidines which are similar in the three derivatives, three others which are similar in the carbonyl and oxy derivatives, and one other (the distal histidine) which has a low pK′ in the former and a normal pK′ in the latter derivative.