Study of the influence of sodium dodecyl sulphate on warfarin–human serum albumin binding using chromatographic retention data

Abstract

The equilibrium saturation chromatographic method has been applied to the study of the influence of sodium dodecyl sulphate (SDS) on warfarin–human serum albumin binding. The ligand retention volume depends upon the association and gives r, the number of bound ligands per mole of macromolecule. Another method for evaluating the influence of SDS upon drug–protein binding is introduced.