Pyrolysis-gas chromatography of proline, hydroxyproline and related peptides

Abstract

On pyrolysis using a Curie point analyser at 770 °C proline and hydroxyproline (cis- and trans-) yielded as their major products a previously unreported fragment, tentatively identified as 1-pyrroline, and pyrrole, respectively. A number of dipeptides and a tripeptide containing these heterocyclic amino acids were also examined. All the degradations were found to be dependent on the pH of the solution that was examined.