Determination of 6-mercaptopurine and related compounds by phosphorescence spectroscopy

Abstract

The phosphorescence properties of 6-mercaptopurine and ten related compounds have been studied in acidic, neutral and alkaline ethanolic glasses at 77 K, and adsorbed on thin layers at 77 K and at room temperature Nanogram amounts of most of the compounds can be detected at 77 K, and submicrogram amounts at room temperature A technique combining thin-layer chromatography and phosphorimetric scanning of the chromatograms has been used to determine trace amounts of the compounds in human blood plasma.