Tritiated peptides. Part 7. Synthesis of [2,5-3H2-His6]-β-corticotrophin-(1–24)-tetracosapeptide

Abstract

The synthesis is described of β-corticotrophin-(1–24)-tetracosapeptide ‡ labelled with tritium in the histidine residue at position 6 at a specific radioactivity of 30 Ci mmol^–1 by reductive dehalogenation of a protected precursor. Evidence for the integrity of the final product is provided by amino-acid analysis, column chromatography, and bioassay, supported by chemical and enzymic analytical data on the protected precursor and the derived free peptide containing di-iodohistidine.