Determination of vitamin D2 in multivitamin tablets by high-performance liquid chromatography

Abstract

A procedure is described for the determination of vitamin D₂ in multi-vitamin tablets. The vitamin is released from the tablets by enzymic digestion, which is followed by solvent extraction and chromatography on a microparticulate silica column. Other fat-soluble vitamins do not interfere with the assay. The problem of defining and quantifying vitamin D₂ in order to correlate results with biological potency is discussed. The procedure is suitable for stability studies.