Spin trapping experiments on nadh analogues and the role of radicals in carbonyl reductions

Abstract

¹ H N.m.r. and gas chromatographic analysis of the reactants and products show that the reaction of N-benzyl-1,4-dihydronicotinamide (NBDN), an analogue of NADH, with pyridine-2-carbaldehyde does not pass through a radical intermediate during either a thermal or a photolytic reaction; photolysis led to the removal of NBDN but not to the production of the reduced substrate and radicals detected with e.s.r. spectroscopy were shown to be artifacts.