Stereochemistry of hydrogen elimination in the enzymic formation of the C-2–C-3 double bond of porphobilinogen
Abstract
Using 5-aminolaevulinate dehydratase from Rhodopseudomonas spheroides it is shown that the tritium located in the pro-S position at C–5 of 5-aminolaevulinate (2) is retained at C–2 of porphobilinogen (3).