Deuteriodeprotonation of methylcytosines by hydroxylamine in D2O solution
Abstract
When hydroxylamine reacts with 1-methyl-cytosine or with 1,3-dimethylcytosine in D2O solution, partial deuteriodeprotonation is observed at position-5 in both products and to a greater extent in methylcytosines which had not reacted thereby showing that hydroxyl-amine addition across the pyrimidine 5,6-double bond is reversible and is only partly stereospecific.