Incorporation of [α-2H]- and [α-3H]-L-cystine into penicillin G and the location of the label using isotope exchange and 2H nuclear magnetic resonance
Abstract
The feeding of L-[α2H]- and L-[α-3H]-cystine to Penicilliun chrysogenum affords [6-2H]- and [6-3H]- penicillin G, thus confirming that an αβ-dehydrocysteine residue is not involved in the biosynthetic pathway.