Carbon-13 nuclear magnetic resonance in biosynthetic studies of lipids
Abstract
13 C-labelled lipids (enrichment 32%) have been isolated from wild-type yeast, Saccharomyces cerevisiae(x2180), grown on media containing sodium [2-13C]-acetate (50–60%); the 13C n.m.r. spectrum of 13C-enriched methyl palmitoleate is correlated with the natural abundance 13C resonance signals assigned using additive bond parameters and structural analogies with reference compounds.