An enzymic method for the determination of lactose in milk including human milk

Abstract

An enzymic method is described for the determination of lactose in milk. The method is based on the determination of free lactose by its hydrolysis with β-galactosidase, the glucose produced being determined by the hexokinase method. The determination is free from interference by other sugars. The method is more accurate and reliable than other methods currently in use. Further, it is applicable to milk samples available only in small amounts.