Synthesis-enabled conformational assignment of natural N-acyl l-phenylalanine derivatives from freshwater sponge–associated Micromonospora sp. MS-62

Abstract

This study demonstrates a concise four-step total synthesis of N-acyl-L-phenylalanine derivatives (P1–P5) from Micromonospora sp. MS-62 enabling definitive assignment of the S configuration at C-1′ via single-crystal X-ray diffraction. This synthesis clarifies the stereochemistry of this natural product class and facilitates future exploration of structure–activity relationship.