Two new prenylated tryptophan analogues isolated from the coral-derived fungus Tritirachium sp. SCSIO 41037

Abstract

Two new prenylated tryptophan analogues, tritianamides A (1) and B (2), together with ten known nitrogen-containing compounds (3–12), were isolated from the ethyl acetate extract of coral-derived fungus Tritirachium sp. SCSIO 41037. The compounds were purified by extraction, silica gel column chromatography and semi-preparative HPLC. Their structures were identified using NMR and HR-ESI-MS data. The absolute configurations of 1 and 2 were determined by the calculated ECD. In bioassay, all compounds were screened for enzyme inhibitory activity against acetylcholinesterase (AChE) in both virtual screening and in vitro assays. The results revealed that 3 and 5 exhibited moderate inhibitory effects, with IC₅₀ values of 0.32 and 0.46 mM, respectively. We also propose a biosynthetic pathway of 1 and 2 originating from tryptophan.