A bis-macrocylic peptide ligand mimicking the β-sandwich active site of multicopper oxidases

Abstract

The active site of multicopper oxidases contains three Cu ions coordinated to a β-sandwich motif. Here, we designed a small peptide ligand that closely mimics the β-sandwich. We verified the sandwich architecture by X-ray crystallography, showing its high resemblance to the active site and ability to coordinate two Cu(II) ions.