Silicon rhodamine-based fluorescent probes for monitoring mitochondrial viscosity dynamics during mitophagy

Abstract

Mitochondrial autophagy (mitophagy) is pivotal for mitochondrial quality control and intracellular homeostasis. However, real-time visualization of mitochondrial inner membrane viscosity – a key biophysical parameter of mitophagy – remains challenging. To address this, a silicon rhodamine (SiR)-based dual-modal imaging probe was developed, enabling reliable real-time monitoring of mitophagic processes and providing novel insights into mitochondrial remodeling during autophagy.