Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

Mizuki Nakashima; Daimei Miura; Erika Komiya; Nanami Watanabe; Kaori Tsukakoshi; Tsutomu Omatsu; Wakako Tsugawa; Koji Sode; Kazunori Ikebukuro; Toshinori Yoshida; Ryutaro Asano

doi:10.1039/D6AY00278A

Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

Mizuki Nakashima,†^a Daimei Miura,

†^ab Erika Komiya,^a Nanami Watanabe,^c Kaori Tsukakoshi,^d Tsutomu Omatsu,

^e Wakako Tsugawa,

^a Koji Sode,

^f Kazunori Ikebukuro,

^a Toshinori Yoshida

^c and Ryutaro Asano

*^a

Author affiliations

* Corresponding authors

^a Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, Japan
E-mail: ryutaroa@cc.tuat.ac.jp

^b Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-8-1 Harumi-cho, Fuchu, Tokyo, Japan

^c Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan

^d Department of Chemistry, Faculty of Science Division I, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan

^e Center for Infectious Disease Epidemiology and Prevention Research, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan

^f Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, Chapel Hill, NC 27599, USA

Abstract

We established a rapid and convenient electrochemical detection system for quantifying mouse fecal hemoglobin to evaluate colitis severity. A human myoglobin-binding aptamer was cross-reactive with mouse hemoglobin, and coupled with redox enzymes, enabled sensitive detection in feces (12.5–200 nM) within 20 min. This highlights the potential of cross-species hemoglobin detection.

Analytical Methods

Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

Abstract

Supplementary files

Article information

Download Citation

Permissions

Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

Social activity

Search articles by author

Spotlight

Advertisements