From the journal:

Analytical Methods

Transwell-based analysis uncovers stimulus-specific chemotactic migration in Dictyostelium discoideum

Miranda Bravo,a   Antonia Ramos-Guzmán,a   Diego Rojas,a   Ian Pérez, ORCID logo a   Andrés E. Marcoleta ORCID logo b  and  Francisco P. Chávez ORCID logo *a  

* Corresponding authors

a Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
E-mail: fpchavez@uchile.cl

b Grupo de Microbiología Integrativa, Laboratorio de Biología Estructural y Molecular, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

Abstract

We report an adapted Transwell® assay for robust and quantitative analysis of chemotactic migration in Dictyostelium discoideum. This interface-based platform enables quantitative discrimination of stimulus-specific chemotactic migration and reveals defective migration in cells lacking a functional chemotactic GPCR, supporting rapid identification of genetic and environmental determinants of amoeboid motility.

Graphical abstract: Transwell-based analysis uncovers stimulus-specific chemotactic migration in Dictyostelium discoideum

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Article information

DOI
https://doi.org/10.1039/D6AY00051G
Article type
Communication
Submitted
10 Jan 2026
Accepted
04 Mar 2026
First published
06 Mar 2026

Anal. Methods, 2026, Advance Article

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Transwell-based analysis uncovers stimulus-specific chemotactic migration in Dictyostelium discoideum

M. Bravo, A. Ramos-Guzmán, D. Rojas, I. Pérez, A. E. Marcoleta and F. P. Chávez, Anal. Methods, 2026, Advance Article , DOI: 10.1039/D6AY00051G

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