Wenjun
Luo
*a,
Xinghua
Zheng
a,
Hehua
Lin
a,
Li
Fu
b,
Lipeng
Long
a,
Daohong
Yu
a,
Zhengwang
Chen
a,
Min
Yang
*b and
Zhong-Xia
Wang
*a
aJiangxi Provincial Key Laboratory of Synthetic Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, P. R. China. E-mail: wenjunluo@gnnu.edu.cn; zhongxiawang@ncu.edu.cn
bSchool of Pharmacy, Jiangxi Provincial Key Laboratory of Tissue Engineering, Gannan Medical University, Ganzhou 341000, China. E-mail: min_yang100@163.com
First published on 28th January 2025
Developing efficient procedures for the synthesis of combinations of pharmacophores continues to be a vital objective in synthetic science. Herein, we report an unprecedented family of dihydrobenzo[b][1,8]naphthyridine-ylidene-pyrrolidinetriones achieved by reacting ortho-halogenated quinolonechalcones with aminomaleimides under metal-free conditions. Among these compounds, several exhibit the potential to serve as fluorescent dyes for biological applications. Mechanistic investigations indicate that the reaction proceeds via a 1,4-Michael addition followed by an intermolecular cascade annulation, which involves aniline fragment transfer and SNAr processes. As far as we know, studies regarding the synthesis of dihydrobenzo[b][1,8]naphthyridine-ylidene-pyrrolidinetriones are rare. This discovery offers great inspiration for a feasible approach toward the creation of more complex and useful molecules.
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Fig. 1 The significance of pyrrolidine-2.5-diones and dihydrobenzo[b][1,8]naphthyridine derivatives. |
Developing efficient procedures for synthesizing combinations of pharmacophores remains a crucial objective in synthetic organic chemistry.7 Consequently, our interest lies in investigating novel strategies or methodologies to synthesize dihydrobenzo[b][1,8]naphthyridines fused with pyrrolidinediones, which could represent promising and innovative approaches in the field of drug discovery. According to the literature, various methodologies for preparing pyrrolidine-2.5-diones8 and dihydrobenzo[b][1,8]-naphthyridine derivatives9 from easily available materials have been well established. It is widely known that aminomaleimides can attack electrophiles at two positions: the carbon atom of the CC group10 and the N-group11 substituted at the 3-position. As illustrated in Scheme 1b: (a) when reacting with a monoelectrophilic reagent, two or more distinct products may potentially form. (b) With an amphiphilic electrophilic reagent, one possible pathway involves reactions at the C-4 position and the N site substituted at the 3-position, leading to the formation of cyclized products.12
Similarly, ortho-halogenated quinolonechalcones can undergo nucleophilic attacks at three positions: the carbon atom of the CO group, the β-carbon of the C
C bond, and the C2′-quinoline site.13 These three active centers are all electron-deficient, making them susceptible to nucleophilic attack. As illustrated in Scheme 1c: (a) when reacting with a mononucleophilic reagent, three or more distinct products may potentially form.14 (b) With an amphiphilic nucleophilic reagent, one possible pathway involves reactions at the C1 and C3 sites, leading to the formation of cyclized products.15 Another pathway may involve reactions at the C2′ and C3 sites, also yielding cyclized products, or additional reaction pathways may exist.16
Building on our previous work,17 we envision that the equilibrium of aminomaleimides indicates its potential as a suitable synthon in the proposed annulation. By using ortho-halogenated quinolonechalcones 1, which contain a Michael acceptor and a chlorine or bromine leaving group at the ortho-position of the quinoline ring, the expected dihydrobenzo[b][1,8]naphthyridines fused with pyrrolidinediones 3′ or 3′′ could be readily achieved (Scheme 1d). However, we did not obtain the anticipated products. Based on previous developments,18 cascade annulation reactions have emerged as a useful strategy for preparing complex molecular structures, especially in metal-free reactions.19 We discovered that the reaction unexpectedly proceeded via a 1,4-Michael addition followed by an intermolecular cascade annulation, resulting in the formation of dihydrobenzo[b][1,8]naphthyridines fused with pyrrolidinetrione, compound 3, under metal-free conditions (Scheme 1a). Hopefully, this work has also been highlighted through gaining valuable insights into fluorescent dyes for potential biological applications.
Initially, we investigated the reaction between 3-((4-bromophenyl)amino)-1-ethyl-1H-pyrrole-2,5-dione 1a and (E)-1-phenyl-3-(phenylamino)prop-2-en-1-one (2a, 2.0 equiv.) in the presence of 1.5 equivalents of cesium carbonate (Cs2CO3) in ethanol at reflux for 12 hours (Table 1). To our delight, the unexpected product (E)-4-(1-(4-bromophenyl)-2-phenylbenzo-[b][1,8]naphthyridin-4(1H)-ylidene)-1-ethylpyrrolidine-2,3,5-trione 3a was obtained in 26% yield (entry 1). Subsequently, various bases were screened (K2CO3, Na2CO3, Et3N, DBU, and DABCO, entries 2–8), but none provided better results than Cs2CO3. The solvent effect was also investigated, and the results showed that N,N-dimethylformamide (DMF) proved to be the best choice, furnishing the desired product 3a in 70% yield (entries 9–15). Next, the addition of oxidizing additives did not lead to the detection of 3a (entries 16–19). When ‘Br’ was substituted for ‘Cl’ at C-2′ in substrate 2a, product 3a was also attained (entry 20). Additionally, decreasing the reaction temperature to 60 °C or replacing cesium carbonate with potassium carbonate failed to provide good yields (Table 1, entries 21 and 22). Consequently, the optimal reaction conditions were identified as using Cs2CO3 as the base and DMF as the solvent at 80 °C.
Entry | Base | Additive | Solvent | Temp (°C) | Yieldb (%) |
---|---|---|---|---|---|
a Reaction conditions: 1a (0.1 mmol) and 2a (0.2 mmol) were reacted at reflux or 80 °C in the presence of base (0.15 mmol) in 2 mL of the solvent in air. b Isolated yields. n.d. = not detected. c ‘Cl’ at C-2′ of 2a instead with ‘Br’. | |||||
1 | Cs2CO3 | — | EtOH | Reflux | 26 |
2 | K2CO3 | — | EtOH | Reflux | 15 |
3 | Na2CO3 | — | EtOH | Reflux | Trace |
4 | Et3N | — | EtOH | Reflux | Trace |
5 | DBU | — | EtOH | Reflux | Trace |
6 | DABCO | — | EtOH | Reflux | Trace |
7 | Piperidine | — | EtOH | Reflux | n.d. |
8 | PPh3 | — | EtOH | Reflux | n.d. |
9 | Cs2CO3 | — | MeOH | Reflux | 12 |
10 | Cs2CO3 | — | EA | Reflux | 10 |
11 | Cs2CO3 | — | Toluene | 80 | 20 |
12 | Cs2CO3 | — | THF | Reflux | 48 |
13 | Cs2CO3 | — | DMSO | 80 | 44 |
14 | Cs 2 CO 3 | — | DMF | 80 | 70 |
15 | Cs2CO3 | — | CH3CN | 80 | 30 |
16 | Cs2CO3 | DDQ | DMF | 80 | n.d. |
17 | Cs2CO3 | K2S2O8 | DMF | 80 | n.d. |
18 | Cs2CO3 | BPO | DMF | 80 | Trace |
19 | Cs2CO3 | IBX | DMF | 80 | Trace |
20c | Cs2CO3 | — | DMF | 80 | 68 |
21 | Cs2CO3 | — | DMF | 60 | 45 |
22 | K2CO3 | — | DMF | 80 | 40 |
Using the optimal reaction conditions, we began to evaluate the scope of various aminomaleimides 1 with (E)-1-phenyl-3-(phenylamino)prop-2-en-1-one 2a (Scheme 2). It was observed that aminomaleimides bearing electron-rich at the N-3 position aryl groups reacted more favorably compared to those with electron-deficient substituents. Specifically, N-4-methoxyphenyl-substituted aminomaleimides yielded product 3g in 74% yield, while the N-4-(trifluoromethyl)phenyl-substituted analogs provided product 3i with a lower yield of 42%. The substituent position on the N-aryl group of acyclic enaminone significantly influences the reaction results. Both meta-substituted (3l–3q) and ortho-substituted (3r–3t) aminomaleimide substrates with N-aryl groups produced the target compounds, albeit with reduced yields. Aminomaleimides featuring disubstituted and trisubstituted aryl groups at the N-3 position also demonstrated effectiveness, generating products 3u–3w in 56–60% yield. Moreover, substrates with 1-naphthyl and benzo[d][1,3]dioxol-5-yl substitutions at the N-3 position yielded the target compounds at 70% (3x) and 47% yield (3y), respectively. Notably, benzene rings substituted at the 4-position with a carbazole or N,N-dimethylphenyl group exhibited good compatibility, resulting in the respective products with 45% (3z) and 46% yield (3aa). It is noteworthy that aminomaleimides, which feature ortho-chiral oxazole-substituted groups at the benzene rings 1ab, resulted in a 1:
1 diastereoisomeric mixture of 3ab with a yield of 38%. This outcome is likely attributed to their asymmetrical structure and the steric hindrance imposed by the ortho-substituted aminomaleimide. Such structural features lead to C–N axis chirality and the presence of racemic atropisomers in their configurations. Additionally, aminomaleimides derived from naphthalene-1,5-diamine (1ac) and the reaction with bis-molecular ortho-chloroquinolinechalcone 2a yielded the desired product 3ac with a 30% yield. Encouragingly, replacing the benzene ring with ethyl led to corresponding products in 52% yield (3ad), while adamantane substitution failed to produce the desired 3ae product, possibly due to significant steric hindrance.
Additional functional group compatibility and substrate scope were examined by varying the substituents at the N-1 positions of aminomaleimides. Substrates carrying benzyl (3af), phenyl (3ag), (R) or (S)-2-hydroxypropyl (3ah), and hydroxyethyl (3ai) groups on nitrogen were well tolerated. Next, the effect of substituents on the quinoline ring of ortho-chloroquinolinechalcones 1 was investigated. Substituents including fluorine and methyl groups on the quinoline ring were compatible with aminomaleimide 2a, facilitating the synthesis of the corresponding products in moderate yields (51% and 60%) (3aj and 3ak). Furthermore, disubstituted aromatic rings and those containing a 1-naphthalene group on the quinoline moiety also produced cyclization products efficiently, with yields between 55% and 64% (3al–3an). Surprisingly, ortho-chloroquinolinechalcones 1a with a methyl substitution on the phenyl group could still afford the desired products with a 45% yield (3ao). However, ortho-chloroquinolin-α,β-unsaturated esters (2h and 2i) failed to produce the expected product, which may be attributed to the ester group being less reactive than the ketone. To broaden the chalcone varieties, 2-halopyridinechalcones 6a and 6b were subjected to testing. The identical target product 7a was successfully obtained, with yields of 14% (for 2-Cl) and 23% (for 2-F), respectively (for more details see S38 and S39 in the ESI†). Additionally, the structure of compound 3 was confirmed by X-ray diffraction analysis of 3a (CCDC 2390992†).20
To gain a deeper understanding of the reaction mechanism, we designed and conducted a series of control experiments. First, to investigate whether the cleavage and reformation of the aniline fragment during the reaction is an intramolecular process, we conducted the following experiments (Scheme 3): (a) compound 1a was treated under standard conditions, and p-bromoaniline was successfully detected as a product. However, when an additional 2a was added to this system, the expected formation of target product 3a was not observed. (b) We attempted to react 2a directly with p-bromoaniline under the same standard conditions, but the desired product, where the aniline substitutes for the chlorine atom, was not obtained. (c) We investigated whether a cross-reaction would occur between 1a and 2a in the presence of p-anisidine. Despite the introduction of p-anisidine, the final product remained 3a, indicating that the expected cross-reaction did not take place. To further confirm this, we added aniline to the reaction mixture of 1g and 2a, and the experimental results showed that the only product formed was 3g (for more details see S38 and S39 in the ESI†). These experiments collectively demonstrate that the reformation of the aniline fragment in this reaction occurs strictly through an intramolecular mechanism. Next, to further elucidate the specific pathway of the reaction, we conducted experiments using 1a and 2a in dichloromethane as the solvent, at room temperature, with Cs2CO3 (1.5 equiv.) as the base. From this setup, we successfully isolated intermediate 4a. Detailed nuclear magnetic resonance (NMR) analysis, including 1H-NMR, 13C-NMR, and two-dimensional NMR spectra, along with high-resolution mass spectrometry (HRMS), confirmed that the reaction initially underwent a Michael addition step, forming intermediate 4a (for more details see S40–S48 in the ESI†). Subsequently, submitting the obtained 4a to the standard reaction conditions allowed us to achieve the final target product 3a (Scheme 3d). This process further validated our understanding of the reaction pathway. The structures of the reactant and the product suggest that one molecule of water might be incorporated into the reaction system. Therefore, the role of water in the reaction was investigated. Interestingly, upon the addition of 3.0 equiv. of H18O to the unproductive anhydrous reaction system, the mixture of 18O-labeled and the normal dihydrobenzo[b][1,8]naphthyridines fused with pyrrolidinetrione product 3b was detected (Scheme 3e). This result demonstrated that a small amount of water participates in the reaction.
Considering that commercial solvent was used directly without drying, we hypothesized a plausible reaction mechanism as shown in Scheme 4. Initially, aminomaleimides 1 and ortho-chloroquinolinechalcones 2a undergo Michael addition, leading to the formation of intermediate I, which can undergo tautomerism and transform into 4. Subsequently, water attacks the imine structure of intermediate I, giving the resulting amine which undergoes a synergistic intramolecular addition-transfer-reaction with the carbonyl group of the chalcone, forming six-membered cyclic intermediate II by a series of imine/hemiaminal formations and a hydrolysis process occurs to form intermediate III, which further tautomerizes and transforms into intermediate IV. An intramolecular SNAr substitution ultimately takes place, resulting in the formation of intermediate V, which then undergoes rotation and oxidation to yield the final product 3.
Among these dihydrobenzo[b][1,8]naphthyridine-ylidene-pyrrolidinetriones, we were surprised to find that 3d, 3g, 3k, 3l, 3n, 3r, 3v, 3ag and 3ak showed significant photophysical properties. As part of a program for developing some multifunctional dyes in biological processes,21–28 we evaluated their photophysical properties in six selected organic solvents with different properties (DMSO, EtOH, toluene, 1,4-dioxane, DCM, and PBS) via a multiwell analysis method. The results revealed that these compounds showed different spectral characteristics in different solvents, such as the fluorescence intensity and emission wavelength. As shown in Fig. 2, the monofluoro-substituted dihydrobenzo[b][1,8]naphthyridine-ylidene-pyrrolidinetriones (3d, 3n, and 3r) exhibited near-infrared photophysical properties in PBS (λex = 420 nm, λem = ∼744 nm), which provided their potential biological application. As expected, some of the dihydrobenzo[b][1,8]naphthyridine-ylidene-pyrrolidinetriones (3d, 3n, 3r, and 3ag) were subjected as fluorescent dyes to live human glioma U251 cell imaging, which preliminarily indicated a positive aspect in biocompatibility and low-toxicity (for more details see S79–S84 in the ESI†). Further biological applications will be reported in due course.
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Fig. 2 Live human glioma U251 cell imaging after 4 h of incubation: (a) FITC channel: λex = 488 nm; (b) bright field; (c) merged image. |
Footnote |
† Electronic supplementary information (ESI) available: Synthetic details, spectroscopic characterization and crystallographic data. CCDC 2390992. For ESI and crystallographic data in CIF or other electronic format see DOI: https://doi.org/10.1039/d4sc07999j |
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