Strategies for the optimisation of troublesome peptide nucleic acid (PNA) sequences

Abstract

Through the use of a pseudo-peptidic backbone, peptide nucleic acids (PNA) mimic the functionality of native nucleic acids while enjoying improved binding affinity and metabolic stability. However, many aspects of the application of PNA to biological and medicinal settings still requires sequence specific optimisation. This review highlights key areas for refinement, including synthesis, tuning of physical properties, cell permeability and analysis, including common strategies for the pracitioner to apply in each area.

Graphical abstract: Strategies for the optimisation of troublesome peptide nucleic acid (PNA) sequences

Transparent peer review

To support increased transparency, we offer authors the option to publish the peer review history alongside their article.

View this article’s peer review history

Article information

Article type
Review Article
Submitted
09 Apr 2025
Accepted
13 May 2025
First published
14 May 2025

Org. Biomol. Chem., 2025, Advance Article

Strategies for the optimisation of troublesome peptide nucleic acid (PNA) sequences

E. E. Watson, Org. Biomol. Chem., 2025, Advance Article , DOI: 10.1039/D5OB00589B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements